1L7V
Bacterial ABC Transporter Involved in B12 Uptake
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.7712 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.990, 106.292, 95.543 |
| Unit cell angles | 90.00, 99.29, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.200 |
| R-factor | 0.262 * |
| Rwork | 0.262 |
| R-free | 0.28600 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.120 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.290 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.067 * | 0.448 * |
| Number of reflections | 30660 | |
| Completeness [%] | 99.5 | 94.3 |
| Redundancy | 8.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 278 | PEG 2000, LDAO, Tris, magnesium nitrate, MPD, D2O, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | Tris | 100 (mM) | pH8.0 |
| 3 | 1 | reservoir | magnesium nitrate | 300 (mM) | |
| 4 | 1 | reservoir | PEG2000 | 21 (%) | |
| 5 | 1 | reservoir | MPD | 0.8 (%) | in D2O |
