1L6M
Neutrophil Gelatinase-associated Lipocalin is a Novel Bacteriostatic Agent that Interferes with Siderophore-mediated Iron Acquisition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 115.078, 115.078, 115.221 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.760 - 2.400 |
R-factor | 0.23376 |
Rwork | 0.232 |
R-free | 0.27100 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qqs |
RMSD bond length | 0.013 * |
RMSD bond angle | 2.000 * |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | EPMR |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | |
High resolution limit [Å] | 2.400 | 2.400 * |
Rmerge | 0.057 * | 0.510 * |
Total number of observations | 312664 * | |
Number of reflections | 30746 * | |
Completeness [%] | 100.0 * | 100 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.5 * | 298 | Goetz, D.H., (2000) Biochemistry, 39, 1935. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 18-22 (%(w/w)) | |
3 | 1 | reservoir | glycerol | 15 (%(v/v)) | |
4 | 1 | reservoir | sodium acetate | 100 (mM) |