1L6J
Crystal structure of human matrix metalloproteinase MMP9 (gelatinase B).
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Detector technology | CCD |
| Detector | BRANDEIS |
| Wavelength(s) | 1.271632, 1.209997, 1.272917 |
| Spacegroup name | P 65 |
| Unit cell lengths | 123.685, 123.685, 89.937 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 2.500 |
| Rwork | 0.187 |
| R-free | 0.22300 * |
| Structure solution method | MAD |
| Starting model (for MR) | 1ck7 |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.077 | 0.194 |
| Number of reflections | 47453 * | 3904 * |
| <I/σ(I)> | 11.5 | 2.4 |
| Completeness [%] | 89.1 | 73.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | sodium chloride, Tris, HEPES, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12.8 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH8.2 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | reservoir | 4.3 (M) | ||
| 5 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
