1L3U
Crystal Structure of Bacillus DNA Polymerase I Fragment product complex with 11 base pairs of duplex DNA following addition of a dTTP and a dATP residue.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-03-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000034 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.429, 93.018, 106.137 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 * - 1.800 |
R-factor | 0.1959 |
Rwork | 0.194 |
R-free | 0.22800 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bdp |
RMSD bond length | 0.005 |
RMSD bond angle | 22.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.073 | 0.362 |
Total number of observations | 358326 * | |
Number of reflections | 79466 * | |
<I/σ(I)> | 10.8 | 1.6 |
Completeness [%] | 79.4 * | 48.4 * |
Redundancy | 3.4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | Ammonium Sulfate, magnesium chloride, MPD, MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)2(SO4) | ||
2 | 1 | 1 | MgCl2 | ||
3 | 1 | 1 | MPD | ||
4 | 1 | 1 | MES | ||
5 | 1 | 2 | MgCl2 | ||
6 | 1 | 2 | MPD | ||
7 | 1 | 2 | MES |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 49 (%sat) | |
3 | 1 | reservoir | 2.5 (%(v/v)) | ||
4 | 1 | reservoir | 100 (mM) | pH5.8 | |
5 | 1 | reservoir | 10 (mM) |