1L3L
Crystal structure of a bacterial quorum-sensing transcription factor complexed with pheromone and DNA
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-08-12 |
Detector | SBC-2 |
Wavelength(s) | 0.9795, 0.9797, 0.95600 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 92.298, 58.370, 127.453 |
Unit cell angles | 90.00, 102.81, 90.00 |
Refinement procedure
Resolution | 39.600 - 1.660 |
Rwork | 0.246 |
R-free | 0.25200 * |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 20.000 * |
Data reduction software | d*TREK |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.720 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.082 | 0.422 |
Number of reflections | 139194 | |
<I/σ(I)> | 21.76 | 2 |
Completeness [%] | 89.1 | 70.9 |
Redundancy | 6.69 | 5.29 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | Joachimiak, A., (1991) Methods Enzymol., 208, 82. * |