1L2X
Atomic Resolution Crystal Structure of a Viral RNA Pseudoknot
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-11-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 30.160, 30.160, 140.278 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.250 |
| R-factor | 0.142 * |
| Rwork | 0.142 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 437d |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.079 | 0.428 |
| Number of reflections | 21462 | |
| <I/σ(I)> | 21.4 | 4.1 |
| Completeness [%] | 99.1 | 98.4 |
| Redundancy | 6.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 25 * | Su, L., (1999) Nature Struct. Biol., 6, 285. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | potassium MOPS | ||
| 2 | 1 | 1 | spermidine | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | sec-butanol |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 4 (mg/ml) | |
| 2 | 1 | drop | 5 (mM) | ||
| 3 | 1 | drop | sec-butanol | 5 (%) | |
| 4 | 1 | drop | potassium MOPS | 100 (mM) | |
| 5 | 1 | drop | spermidine | 2 (mM) | |
| 6 | 1 | reservoir | sec-butanol | 18 (%) |
