1L0F

X-ray Crystal Structure of AmpC N152H Mutant beta-Lactamase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-06
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	118.936, 76.260, 97.922
Unit cell angles	90.00, 115.69, 90.00

Refinement procedure

Resolution	20.000 - 1.660
Rwork	0.178
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fsy
RMSD bond length	0.014
RMSD bond angle	1.750 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.700
High resolution limit [Å]	1.660	1.660
Rmerge	0.060	0.217
Total number of observations	369646 *
Number of reflections	90254
<I/σ(I)>	32.8	5.3
Completeness [%]	97.0	94.4
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.7	296	used to seeding, Usher, K.C., (1998) Biochemistry, 37, 16082. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium potassium phosphate	1.7 (M)	pH8.7
2	1	drop	protein	10 (mg/ml)