1L0E
X-ray Crystal Structure of AmpC K67Q Mutant beta-Lactamase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-01-23 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.022, 76.077, 98.094 |
Unit cell angles | 90.00, 115.84, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
Rwork | 0.159 |
R-free | 0.19200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fsy |
RMSD bond length | 0.014 |
RMSD bond angle | 1.730 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.075 | 0.322 |
Total number of observations | 243730 * | |
Number of reflections | 62018 | |
<I/σ(I)> | 15.3 | 2.3 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 296 | used to seeding, Usher, K.C., (1998) Biochemistry, 37, 16082. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium potassium phosphate | 1.7 (M) | pH8.7 |
2 | 1 | drop | protein | 10 (mg/ml) |