1KZY
Crystal Structure of the 53bp1 BRCT Region Complexed to Tumor Suppressor P53
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 1999-06-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.2832,1.2817,1.2622 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.132, 94.976, 133.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.216 * |
| Rwork | 0.216 |
| R-free | 0.25600 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.730 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 30624 | |
| Completeness [%] | 91.7 | |
| Redundancy | 16.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 * | 4 * | PEG4000, sodium citrate, ammonium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 50 (mM) | pH6.0 |
| 2 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 16 (%(w/v)) | |
| 4 | 1 | drop | protein | 13 (mg/ml) | |
| 5 | 1 | drop | BTP-HCl | 50 (mM) | pH6.8 |
| 6 | 1 | drop | 200 (mM) | ||
| 7 | 1 | drop | dithiothreitol | 5 (mM) |
