1KZU

INTEGRAL MEMBRANE PERIPHERAL LIGHT HARVESTING COMPLEX FROM RHODOPSEUDOMONAS ACIDOPHILA STRAIN 10050

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	1994-12-01
Detector	MAR scanner 300 mm plate
Spacegroup name	H 3 2
Unit cell lengths	120.300, 120.300, 296.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 2.500
R-factor	0.227
Rwork	0.227
R-free	0.25300 *
Structure solution method	MIRAS
RMSD bond length	0.019 *
RMSD bond angle	19.800 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.050	0.239
Number of reflections	28574
<I/σ(I)>	5.9	1.9
Completeness [%]	98.9	100
Redundancy	2.3	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	9.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	phosphate	0.9 (M)
2	1	drop	benzamidine hydrochloride	3 (%)
3	1	reservoir	ammonium sulfate	2.4 (M)