1KZN
Crystal Structure of E. coli 24kDa Domain in Complex with Clorobiocin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 140 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-10-09 |
| Wavelength(s) | 0.938 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.714, 47.629, 111.599 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.790 - 2.300 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.28000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.000 | 2.300 |
| Rmerge | 0.043 * | |
| Number of reflections | 16938 | |
| Completeness [%] | 85.0 | 90 |
| Redundancy | 2.86 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | PEG 20000,propanol-2,HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | inhibitor | 0.5 (mM) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 4 | 1 | reservoir | PEG20000 | 24 (%) | |
| 5 | 1 | reservoir | propanol-2 | 12 (%) |
