1KY2

GPPNHP-BOUND YPT7P AT 1.6 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-10
Detector	MARRESEARCH
Wavelength(s)	0.934
Spacegroup name	P 21 21 21
Unit cell lengths	40.137, 60.667, 73.537
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.600
R-factor	0.183 *
Rwork	0.183
R-free	0.23900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ek0
RMSD bond length	0.008 *
RMSD bond angle	1.824 *
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.700
High resolution limit [Å]	1.600	1.570 *
Rmerge	0.103 *	0.181 *
Total number of observations	124357 *
Number of reflections	23450
<I/σ(I)>	8.1	2.7
Completeness [%]	96.2	95.7
Redundancy	5.15	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	20 *	PEG, TRIS, GppNHp, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7-9 (mg/ml)
2	1	reservoir		1 (M)
3	1	reservoir	PEG6000	20-30 (%)