1KXU
CYCLIN H, A POSITIVE REGULATORY SUBUNIT OF CDK ACTIVATING KINASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1996-03-21 |
| Detector | FUJI FILM |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 84.080, 84.080, 374.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.29500 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.780 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.600 * |
| Rmerge | 0.048 * |
| Total number of observations | 71856 * |
| Number of reflections | 18913 |
| Completeness [%] | 85.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | cyclin H | 7 (mg/ml) | |
| 10 | 1 | reservoir | lithium sulohate | 800 (mM) | |
| 11 | 1 | reservoir | ammonium sulphate | 400 (mM) | |
| 12 | 1 | reservoir | citrate | 100 (mM) | |
| 2 | 1 | drop | sodium chloride | 200 (mM) | |
| 3 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | DTT | 1 (mM) | |
| 6 | 1 | drop | HEPES | 10 (mM) | |
| 7 | 1 | drop | lithium sulohate | 800 (mM) | |
| 8 | 1 | drop | ammonium sulphate | 400 (mM) | |
| 9 | 1 | drop | citrate | 100 (mM) |
