1KXG
The 2.0 Ang Resolution Structure of BLyS, B Lymphocyte Stimulator.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 108 |
| Detector technology | CCD |
| Collection date | 2000-07-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.942 |
| Spacegroup name | P 65 |
| Unit cell lengths | 123.580, 123.580, 161.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 * - 2.000 |
| R-factor | 0.189 * |
| Rwork | 0.189 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d0g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.083 * | 0.384 * |
| Total number of observations | 1940104 * | |
| Number of reflections | 93234 | 9746 * |
| <I/σ(I)> | 23.4 | 3.8 |
| Completeness [%] | 99.3 | 93.8 |
| Redundancy | 20.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 279 | 25% dioxane, 25 mM MgCl2, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 279.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 25 (mM) | |
| 3 | 1 | drop | 125 (mM) | pH6. | |
| 4 | 1 | drop | dioxane | 25 (%(v/v)) | |
| 5 | 1 | drop | 25 (mM) | ||
| 6 | 1 | reservoir | dioxane | 25 (%) | |
| 7 | 1 | reservoir | 25 (mM) |
