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1KX5

X-Ray Structure of the Nucleosome Core Particle, NCP147, at 1.9 A Resolution

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID14-4
Synchrotron siteESRF
BeamlineID14-4
Temperature [K]103
Detector technologyIMAGE PLATE
Collection date1999-05-18
DetectorMARRESEARCH
Wavelength(s)0.93
Spacegroup nameP 21 21 21
Unit cell lengths105.950, 181.170, 109.490
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution6.000 - 1.940
R-factor0.209
Rwork0.208
R-free0.27500
Structure solution methodFOURIER SYNTHESIS
Starting model (for MR)1aoi
RMSD bond length0.009
RMSD bond angle1.240

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.010
High resolution limit [Å]1.9401.940
Rmerge0.0720.291
Number of reflections151140
Completeness [%]95.688
Redundancy5.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

6293manganese chloride, potassium chloride, potassium cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111MgCl2
211KCl
311potassium cacodylate
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein4 (mg/ml)
21drop70-85 (mM)
31drop50-60 (mM)
41droppotassium cacodylate20 (mM)pH6.0
51reservoir40-46 (mM)
61reservoir30-40 (mM)
71reservoirpotassium cacodylate10-20 (mM)

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