1KX4
X-Ray Structure of the Nucleosome Core Particle, NCP146b, at 2.6 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID09 |
Synchrotron site | ESRF |
Beamline | ID09 |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 1996-06-02 |
Detector | MARRESEARCH |
Wavelength(s) | 0.85 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.300, 175.690, 109.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.600 |
R-factor | 0.247 |
Rwork | 0.246 |
R-free | 0.30000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aoi |
RMSD bond length | 0.006 |
RMSD bond angle | 0.960 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.072 | 0.157 |
Number of reflections | 60481 | |
Completeness [%] | 89.5 | 45.7 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 | 293 | manganese chloride, potassium chloride, potassium cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MGCl2 | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | potassium cacodylate |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | drop | 70-85 (mM) | ||
3 | 1 | drop | 50-60 (mM) | ||
4 | 1 | drop | potassium cacodylate | 20 (mM) | pH6.0 |
5 | 1 | reservoir | 40-46 (mM) | ||
6 | 1 | reservoir | 30-40 (mM) | ||
7 | 1 | reservoir | potassium cacodylate | 10-20 (mM) |