1KWR
HUMAN CARBONIC ANHYDRASE II COMPLEXED WITH INHIBITOR 0134-36
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 2000-05-18 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.560, 41.400, 72.340 |
Unit cell angles | 90.00, 104.40, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.300 * |
Rwork | 0.196 |
R-free | 0.26900 * |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.443 * |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 |
High resolution limit [Å] | 2.300 * |
Number of reflections | 10124 * |
Completeness [%] | 95.2 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 6 * | tris, nacl, ammonium sulfate, MeHgAc, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.3 (mM) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | 20 (mM) | pH8.0 | |
4 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
5 | 1 | reservoir | 300 (mM) | ||
6 | 1 | reservoir | ammonium sulfate | 27 (M) | |
7 | 1 | reservoir | 5 (mg) |