1KVK
The Structure of Binary complex between a Mammalian Mevalonate Kinase and ATP: Insights into the Reaction Mechanism and Human Inherited Disease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 277 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 78.630, 118.700, 42.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.000 * - 2.400 |
R-factor | 0.217 * |
Rwork | 0.217 |
R-free | 0.26600 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.550 | 2.490 |
High resolution limit [Å] | 2.400 * | 2.400 |
Rmerge | 0.054 * | 0.305 * |
Total number of observations | 30833 * | |
Number of reflections | 14000 * | 1011 * |
Completeness [%] | 86.9 | 77.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 4 * | Hepes, MgCl2, ATP, PEG-5KMME, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13 (mg/ml) | |
2 | 1 | drop | ATP | 1 (mM) | |
3 | 1 | drop | 2 (mM) | ||
4 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
5 | 1 | reservoir | PEG5000 MME | 17.5 (%) |