1KSJ
Complex of Arl2 and PDE delta, Crystal Form 2 (SeMet)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID13 |
Synchrotron site | ESRF |
Beamline | ID13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-07-09 |
Detector | MARRESEARCH |
Wavelength(s) | 0.964 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.815, 65.726, 103.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.800 - 2.600 |
R-factor | 0.214 |
Rwork | 0.209 |
R-free | 0.26400 |
Structure solution method | combination of Molecular Replacement and SAD phasing |
Starting model (for MR) | human Arf1-GppNHp N-terminally truncated by 17 residues (J. Goldberg Cell. 95:237-248 1998) |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.800 | 2.650 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 9926 | |
<I/σ(I)> | 25.1 | 7 |
Completeness [%] | 99.6 | 100 |
Redundancy | 8.7 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 5000 MME, HEPES, MgCl2, beta mercaptoethanol, GTP, PEG 400 as cryo protectant, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |