1KSH

Complex of Arl2 and PDE delta, Crystal Form 2 (native)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-23
Detector	MARRESEARCH
Wavelength(s)	1.005
Spacegroup name	P 21 21 21
Unit cell lengths	44.454, 65.395, 104.409
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.700 * - 1.800
R-factor	0.244 *
Rwork	0.244
R-free	0.27100
Structure solution method	combination of Molecular Replacement and SAD phasing
Starting model (for MR)	ARL3-GDP pdb entry 1fzq
RMSD bond length	0.007
RMSD bond angle	24.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.700	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.042 *	0.316 *
Number of reflections	28815
<I/σ(I)>	38.8	4.8
Completeness [%]	99.5	98.9
Redundancy	8.6	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	Renault, L., (2001) Acta Crystallogr., Sect.D, 57, 1167. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG5000 MME	9-12 (%)
2	1	reservoir	HEPES	0.1 (M)	pH7.0