1KS9
Ketopantoate Reductase from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-08-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.870 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 103.732, 103.732, 55.737 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.28900 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.043 | 0.346 |
| Number of reflections | 32249 | |
| <I/σ(I)> | 3.1 | |
| Completeness [%] | 94.9 | 74.7 |
| Redundancy | 5.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | PEG 4000, Tris, sodium acetate, pH 9.4, VAPOR DIFFUSION, HANGING DROP at 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | pH8.0 |
| 3 | 1 | reservoir | PEG4000 | 28-38 (%) | |
| 4 | 1 | reservoir | Tris | 0.1 (M) | pH9.4 |
| 5 | 1 | reservoir | 0.25 (M) |
