1KPL
Crystal Structure of the ClC Chloride Channel from S. typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-03 |
Detector | BRANDEIS |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 185.719, 90.159, 80.753 |
Unit cell angles | 90.00, 98.95, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.255 |
Rwork | 0.254 |
R-free | 0.28800 * |
Structure solution method | SIRAS |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (0.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.065 | 0.544 |
Number of reflections | 50567 | |
<I/σ(I)> | 13.7 | 1.7 |
Completeness [%] | 94.7 | 96.6 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 20 * | PEG 400, acetate, sodium sulfate, lithium sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-15 (mg/ml) | |
2 | 1 | drop | n-octyl-beta-maltoside | 45 (mM) | |
3 | 1 | drop | 75 (mM) | ||
4 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
5 | 1 | reservoir | PEG400 | 26-31 (%) | |
6 | 1 | reservoir | sodium acetate | 50 (mM) | pH4.6 |
7 | 1 | reservoir | 75-100 (mM) | ||
8 | 1 | reservoir | 75-100 (mM) |