1KOL
Crystal structure of formaldehyde dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 85.686, 85.686, 190.752 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.650 |
| Rwork | 0.171 |
| R-free | 0.20600 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.025 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.065 | 0.273 |
| Number of reflections | 94085 | |
| <I/σ(I)> | 8.2 | 2.8 |
| Completeness [%] | 97.4 | 83.9 |
| Redundancy | 10 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Tris, ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PFDH | 20 (mg/ml) | |
| 2 | 1 | drop | NAD+ | 4 (mg/ml) | |
| 3 | 1 | drop | Tris | 0.02 (M) | pH8.0 |
| 4 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 5 | 1 | reservoir | dioxane | 10 (%(v/v)) | |
| 6 | 1 | reservoir | Tris | 0.1 (M) | pH8.0 |
