1KOE

ENDOSTATIN

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-11-17
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	45.560, 53.950, 65.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.500
R-factor	0.19
Rwork	0.190
R-free	0.22400
Structure solution method	MIRAS AND SOLVENT FLATTENING AT 2.2 A RESOLUTION
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.075	0.180
Total number of observations	159011 *
Number of reflections	26471
<I/σ(I)>	5.5	3.9
Completeness [%]	99.3	99.2
Redundancy	5.9	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.3		drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	Mops	5 (mM)
3	1	reservoir	ammonium sulfate	1.5-1.7 (M)