1KO0

Crystal Structure of a D,L-lysine complex of diaminopimelate decarboxylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	98
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	98.997, 98.997, 176.966
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.200
Rwork	0.181
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1knw
RMSD bond length	0.014
RMSD bond angle	0.014
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.170
Rmerge	0.073
Completeness [%]	97.8
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	Lithium sulfate, MES, pyridoxal-5'-phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K