1KMZ
MOLECULAR BASIS OF MITOMYCIN C RESICTANCE IN STREPTOMYCES: CRYSTAL STRUCTURES OF THE MRD PROTEIN WITH AND WITHOUT A DRUG DERIVATIVE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-07-24 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 44.508, 60.651, 43.776 |
Unit cell angles | 90.00, 105.97, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.500 |
R-factor | 0.163 |
R-free | 0.23400 * |
Starting model (for MR) | 1kll |
RMSD bond length | 0.016 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.034 | 0.335 * |
Total number of observations | 74125 * | |
Number of reflections | 17820 | |
Completeness [%] | 99.9 | 99.4 * |
Redundancy | 4.15 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6 | 298 | ammonium sulfate, MES buffer, beta-octylglucoside, pH 6.0, VAPOR DIFFUSION at 298K, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 3 (mg/ml) | |
2 | 1 | 1 | ammonium sulfate | 55 (%) | |
3 | 1 | 1 | MES | 100 (mM) | pH6.0 |