1KL9
Crystal structure of the N-terminal segment of Human eukaryotic initiation factor 2alpha
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-12-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9795, 0.9793, 0.9640 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 37.280, 44.200, 121.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.19969 |
Rwork | 0.198 |
R-free | 0.23264 |
Structure solution method | MAD |
RMSD bond length | 0.011 * |
RMSD bond angle | 1.190 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 * |
High resolution limit [Å] | 1.900 |
Rmerge | 0.060 |
Number of reflections | 14922 |
<I/σ(I)> | 7.7 |
Completeness [%] | 90.9 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 22 * | PEG 4000, sodium acetate, zinc chloride, pH 5.5, VAPOR DIFFUSION, SITTING DROP at 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 10-16 (%) | |
3 | 1 | reservoir | sodium acetate | 100 (mM) | |
4 | 1 | reservoir | 0.2 (M) |