1KL7

Crystal Structure of Threonine Synthase from Yeast

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-28
Detector	MARRESEARCH
Wavelength(s)	0.92, 0.98, 0.9874
Spacegroup name	P 1 21 1
Unit cell lengths	95.300, 51.600, 106.900
Unit cell angles	90.00, 99.60, 90.00

Refinement procedure

Resolution	8.000 * - 2.700
R-factor	0.212 *
Rwork	0.201
R-free	0.25200 *
Structure solution method	MAD
RMSD bond length	0.008 *
RMSD bond angle	1.350 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.063	0.172
Total number of observations	145806 *
Number of reflections	54312 *
<I/σ(I)>	16.8	4.6
Completeness [%]	98.8	97.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	4 *	Hepes/NaOH, PEG4000, Dioxan, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	HEPES-NaOH	0.1 (M)	pH7.6
3	1	reservoir	PEG4000	20 (%)
4	1	reservoir	dioxane	10 (%)