1KGY

Crystal Structure of the EphB2-ephrinB2 complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	123
Detector technology	CCD
Collection date	2000-09-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	78.000, 78.000, 78.000
Unit cell angles	69.00, 75.00, 69.00

Refinement procedure

Resolution	25.000 - 2.700
R-factor	0.243 *
Rwork	0.243
R-free	0.29500 *
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.637
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.820
High resolution limit [Å]	2.700	2.700
Rmerge	0.073	0.370
Number of reflections	42156
Completeness [%]	96.8	93.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2 *	20 *	1.6 M Na/K Phosphate, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop		10 (mM)
3	1	drop		2 (mM)
4	1	drop	HEPES	10 (mM)	pH7.2
5	1	reservoir	sodium potassium phospahte	1.6 (M)
6	1	reservoir	HEPES-NaOH	100 (mM)	pH7.5