1KGY
Crystal Structure of the EphB2-ephrinB2 complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2000-09-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.000, 78.000, 78.000 |
| Unit cell angles | 69.00, 75.00, 69.00 |
Refinement procedure
| Resolution | 25.000 - 2.700 |
| R-factor | 0.243 * |
| Rwork | 0.243 |
| R-free | 0.29500 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.637 |
| Data reduction software | MAR |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.820 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.073 | 0.370 |
| Number of reflections | 42156 | |
| Completeness [%] | 96.8 | 93.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 20 * | 1.6 M Na/K Phosphate, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | HEPES | 10 (mM) | pH7.2 |
| 5 | 1 | reservoir | sodium potassium phospahte | 1.6 (M) | |
| 6 | 1 | reservoir | HEPES-NaOH | 100 (mM) | pH7.5 |
