1KF9
PHAGE DISPLAY DERIVED VARIANT OF HUMAN GROWTH HORMONE COMPLEXED WITH TWO COPIES OF THE EXTRACELLULAR DOMAIN OF ITS RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 193 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.289, 111.937, 95.290 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.32600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.800 |
| Data reduction software | MARSCALE |
| Data scaling software | MARSCALE |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.049 | 0.210 |
| Total number of observations | 215604 * | |
| Number of reflections | 43162 | |
| <I/σ(I)> | 23 | 4.2 |
| Completeness [%] | 95.9 | 78.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.3 | pH 6.30 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 4 | 1 | reservoir | PEG2500 | 30 (%) | pH6.3 |
