1KF5
Atomic Resolution Structure of RNase A at pH 7.1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.440, 38.380, 53.320 |
Unit cell angles | 90.00, 105.74, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.150 |
R-factor | 0.106 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7rsa |
RMSD bond length | 0.015 |
RMSD bond angle | 0.033 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.170 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.043 | 0.307 |
Number of reflections | 42235 * | |
<I/σ(I)> | 12.1 | 3.7 |
Completeness [%] | 99.9 | 98 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 7.1 | 20 * | Tilton Jr., R.F., (1992) Biochemistry, 31, 2469. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 12 (mg/ml) | |
2 | 1 | 1 | 0.2 (M) | ||
3 | 1 | 1 | methanol | 50 (%(v/v)) |