1KF3
Atomic Resolution Structure of RNase A at pH 5.9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.440, 38.380, 53.320 |
| Unit cell angles | 90.00, 105.74, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7rsa |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.070 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.330 | 0.345 |
| Number of reflections | 53494 * | |
| <I/σ(I)> | 18.9 | 4.5 |
| Completeness [%] | 96.4 | 92.9 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5.9 | 20 * | Tilton Jr., R.F., (1992) Biochemistry, 31, 2469. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 12 (mg/ml) | |
| 2 | 1 | 1 | 0.2 (M) | ||
| 3 | 1 | 1 | methanol | 50 (%(v/v)) |
