1KF2

Atomic Resolution Structure of RNase A at pH 5.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	30.440, 38.380, 53.320
Unit cell angles	90.00, 105.74, 90.00

Refinement procedure

Resolution	30.000 - 1.100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rsa
RMSD bond length	0.015
RMSD bond angle	0.038
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.120
High resolution limit [Å]	1.100	1.100
Rmerge	0.034	0.329
Number of reflections	46613 *
<I/σ(I)>	16	4.1
Completeness [%]	96.7	89.4
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	5.2	20 *	Tilton Jr., R.F., (1992) Biochemistry, 31, 2469. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	12 (mg/ml)
2	1	1		0.2 (M)
3	1	1	methanol	50 (%(v/v))