Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

1KEG

Antibody 64M-2 Fab complexed with dTT(6-4)TT

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsMACSCIENCE
Temperature [K]277
Detector technologyIMAGE PLATE
Collection date1998-01-01
DetectorRIGAKU RAXIS IV
Wavelength(s)1.5418
Spacegroup nameP 21 21 21
Unit cell lengths73.567, 137.274, 49.615
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution10.000 - 2.400
R-factor0.207
Rwork0.201
R-free0.26800
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1ehl
RMSD bond length0.008
RMSD bond angle1.440
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareX-PLOR
Refinement softwareX-PLOR (3.843)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]29.6002.490
High resolution limit [Å]2.4002.400
Rmerge0.0770.304
Number of reflections17570
<I/σ(I)>21.83
Completeness [%]92.085.8
Redundancy54.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8.5293PEG 8000, nickel chloride, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111PEG 8000
211NiCl2
311Tris-HCl

226707

PDB entries from 2024-10-30

PDB statisticsPDBj update infoContact PDBjnumon