1KEG
Antibody 64M-2 Fab complexed with dTT(6-4)TT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1998-01-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.567, 137.274, 49.615 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.400 |
R-factor | 0.207 |
Rwork | 0.201 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ehl |
RMSD bond length | 0.008 |
RMSD bond angle | 1.440 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.600 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.077 | 0.304 |
Number of reflections | 17570 | |
<I/σ(I)> | 21.8 | 3 |
Completeness [%] | 92.0 | 85.8 |
Redundancy | 5 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 8000, nickel chloride, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8000 | ||
2 | 1 | 1 | NiCl2 | ||
3 | 1 | 1 | Tris-HCl |