1KBI
Crystallographic Study of the Recombinant Flavin-binding Domain of Baker's Yeast Flavocytochrome b2: Comparison with the Intact Wild-type Enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 164.160, 164.160, 111.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.17 * |
| Rwork | 0.170 |
| R-free | 0.21100 |
| Starting model (for MR) | 1fcb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.900 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.043 | 0.207 * |
| Total number of observations | 157962 * | |
| Number of reflections | 65248 * | |
| <I/σ(I)> | 25.6 | 4.8 |
| Completeness [%] | 90.5 | 73.7 |
| Redundancy | 2.2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.2 | 4 * | phosphate buffer, D,L-lactate, EDTA, MPD, pH 7.2, MICRODIALYSIS, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 5-7.5 (mg/ml) | |
| 2 | 1 | 1 | phosphate | 50 (mM) | pH7.2 |
| 3 | 1 | 1 | D,L-lactate | 50 (mM) | |
| 4 | 1 | 1 | EDTA | 1 (mM) | |
| 5 | 1 | 2 | MPD | 30-33 (%(v/v)) | |
| 6 | 1 | 2 | phosphate | 70 (mM) | pH7.2 |
| 7 | 1 | 2 | D,L-lactate | 47 (mM) | |
| 8 | 1 | 2 | EDTA | 1 (mM) |
