1KAO
CRYSTAL STRUCTURE OF THE SMALL G PROTEIN RAP2A WITH GDP
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.826, 35.315, 58.356 |
| Unit cell angles | 90.00, 106.67, 90.00 |
Refinement procedure
| Resolution | ? - 1.700 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RAS-GDP |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.071 |
| Number of reflections | 15836 |
| Completeness [%] | 92.8 |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | PEG 6000 (25%), TRIS 100MM PH 8, MGCL2 100MM, pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | EDTA | 5 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 19 (%) | |
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) |
