1K9Z
The PAPase Hal2p complexed with zinc ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-08-12 |
| Detector | BRANDEIS |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.682, 45.359, 71.280 |
| Unit cell angles | 90.00, 111.40, 90.00 |
Refinement procedure
| Resolution | 65.940 - 1.500 |
| R-factor | 0.16112 |
| Rwork | 0.160 |
| R-free | 0.19048 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qgx |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.740 | 1.539 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.090 | 0.430 |
| Number of reflections | 50496 | |
| <I/σ(I)> | 14.3 | 3.5 |
| Completeness [%] | 96.4 | 96.5 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30 % PEG5000 MME, 0.1 M sodium acetate, 5 mM beta-mercaptoethanol, 0.1 M MES. (Crystals were soaked in mother liquor containing 0.5 M zinc chloride for 5 hours.) , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
