1K9A

Crystal structure analysis of full-length carboxyl-terminal Src kinase at 2.5 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-09
Detector	OXFORD PX210
Wavelength(s)	0.900
Spacegroup name	P 21 21 21
Unit cell lengths	115.000, 162.600, 232.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.500 - 2.500
R-factor	0.246
Rwork	0.246
R-free	0.28800
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	1.427
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.000 *	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.073	0.398
Number of reflections	150489	17029 *
<I/σ(I)>	6.9	1.7
Completeness [%]	98.2	98.2
Redundancy	5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	288	ammonium sulphate, HEPES buffer, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 288K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	HEPES-NaOH	50 (mM)	pH7.4
3	1	reservoir	ammonium sulfate	1.90-1.95 (M)