1K78

Pax5(1-149)+Ets-1(331-440)+DNA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-08
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9788, 0.9790, 0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	77.840, 89.750, 170.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.100 * - 2.250
R-factor	0.229 *
Rwork	0.227
R-free	0.25700 *
Structure solution method	MAD
RMSD bond length	0.006 *
RMSD bond angle	1.120 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.940	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.066	0.198
Number of reflections	57710
<I/σ(I)>	21.8	8
Completeness [%]	100.0 *	99.8 *
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	50mM magnesium acetate, 200mM ammonium sulphate, 20% PEG4000, 100mM acetate buffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	50mM magnesium acetate, 200mM ammonium sulphate, 20% PEG4000, 100mM acetate buffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	magnesium acetate
2	1	1	ammonium sulphate
3	1	1	PEG4000
4	1	1	acetate buffer

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	DNA	0.34 (mM)
2	1	reservoir	magnesium acetate	50 (mM)
3	1	reservoir	ammonium sulfate	200 (mM)
4	1	reservoir		100 (mM)	pH4.6
5	1	reservoir	PEG4000	20 (%)