1K6M
Crystal Structure of Human Liver 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.980, 185.330, 89.670 |
| Unit cell angles | 90.00, 90.70, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bif |
| RMSD bond length | 0.011 |
| RMSD bond angle | 29.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.400 | |
| Rmerge | 0.074 | |
| Number of reflections | 42142 | |
| <I/σ(I)> | 20.1 | |
| Completeness [%] | 95.5 | 94.4 * |
| Redundancy | 3.9 | 3.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.25 * | PEG 4000, Tris, sodium phosphate, , pH 8.0, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 10 | 1 | reservoir | ethylene glycol | 20-25 (%) | |
| 11 | 1 | reservoir | PEG4000 | 8 (%) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.25 |
| 3 | 1 | drop | NaPi | 10 (mM) | |
| 4 | 1 | drop | glycerol | 15 (%) | |
| 5 | 1 | drop | EDTA | 0.5 (mM) | |
| 6 | 1 | drop | dithiothreitol | 10 (mM) | |
| 7 | 1 | drop | ATP gammaS/Mg | 6 (mM) | or ADP |
| 8 | 1 | drop | Fru-6-P | 5-10 (mM) | |
| 9 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.5 |
