1K5G

Crystal structure of Ran-GDP-AlFx-RanBP1-RanGAP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-11
Detector	MARRESEARCH
Wavelength(s)	0.934
Spacegroup name	P 1
Unit cell lengths	99.930, 102.568, 118.850
Unit cell angles	71.67, 79.09, 67.81

Refinement procedure

Resolution	31.000 - 3.100
R-factor	0.25 *
Rwork	0.250
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k5d
RMSD bond length	0.008
RMSD bond angle	1.400
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.300
High resolution limit [Å]	3.100	3.100
Rmerge	0.166	0.235
Total number of observations	207495 *
Number of reflections	92906
<I/σ(I)>	3	1.7
Completeness [%]	96.0	95
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	20 *	PEG4000, TRIS-HCL, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		2 (mM)
2	1	drop		30 (mM)
3	1	drop	Tris-HCl	20 (mM)	pH7.5
4	1	drop		2 (mM)
5	1	drop	DTE	2 (mM)
6	1	reservoir	PEG4000	18.5-20.5 (%)
7	1	reservoir	Tris-HCl	100 (mM)	pH7.5