1K3V

Porcine Parvovirus Capsid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	P 21 21 2
Unit cell lengths	442.490, 443.050, 251.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.500
R-factor	0.275 *
Rwork	0.286
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Feline Panleukopenia virus
RMSD bond length	0.010
RMSD bond angle	26.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE (+ DM)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.620
High resolution limit [Å]	3.500	3.500
Rmerge	0.290	0.574
Number of reflections	254870
<I/σ(I)>	1.8	0.9
Completeness [%]	41.3	28.6
Redundancy	1.46	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	18 *	20mM Hepes-NaOH 8mM CaCl, .05% sodium azide, 1-1.5% PEG8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	2	HEPES-NaOH	20 (mM)	pH6.5
3	1	2		8 (mM)
4	1	2	sodium azide	0.05 (%(w/v))
5	1	2	PEG8000	1.0	or 1.5%