1K3F

Uridine Phosphorylase from E. coli, Refined in the Monoclinic Crystal Lattice

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	293
Detector technology	DIFFRACTOMETER
Collection date	1994-01-05
Detector	KARD-6
Spacegroup name	P 1 21 1
Unit cell lengths	92.600, 98.800, 93.700
Unit cell angles	90.00, 120.20, 90.00

Refinement procedure

Resolution	6.000 - 2.500
Rwork	0.186
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	trigonal form of Upase
RMSD bond length	0.012
RMSD bond angle	2.095
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.500
Rmerge	0.097
Total number of observations	116050 *
Number of reflections	42403
Completeness [%]	84.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	293	drops-0.05 M Tris-HCl and 4-6% PEG 4000; equilibrium solution-0.1M Tris-mal/NaOH pH5.91-5.96, 20-25% Peg 4000 and 0.04% sodium azide, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	0.05 (M)
2	1	drop	protein	10-12 (mg/ml)
3	1	drop	PEG4000	4-6 (%)
4	1	reservoir	Tris-mal/NaOH	0.1 (M)
5	1	reservoir	PEG4000	20-25 (%)
6	1	reservoir	sodium azide	0.04 (%)