1K3F
Uridine Phosphorylase from E. coli, Refined in the Monoclinic Crystal Lattice
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | OTHER |
Temperature [K] | 293 |
Detector technology | DIFFRACTOMETER |
Collection date | 1994-01-05 |
Detector | KARD-6 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 92.600, 98.800, 93.700 |
Unit cell angles | 90.00, 120.20, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.500 |
Rwork | 0.186 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | trigonal form of Upase |
RMSD bond length | 0.012 |
RMSD bond angle | 2.095 |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.097 |
Total number of observations | 116050 * |
Number of reflections | 42403 |
Completeness [%] | 84.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | drops-0.05 M Tris-HCl and 4-6% PEG 4000; equilibrium solution-0.1M Tris-mal/NaOH pH5.91-5.96, 20-25% Peg 4000 and 0.04% sodium azide, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 0.05 (M) | |
2 | 1 | drop | protein | 10-12 (mg/ml) | |
3 | 1 | drop | PEG4000 | 4-6 (%) | |
4 | 1 | reservoir | Tris-mal/NaOH | 0.1 (M) | |
5 | 1 | reservoir | PEG4000 | 20-25 (%) | |
6 | 1 | reservoir | sodium azide | 0.04 (%) |