1K2W
Crystal structure of sorbitol dehydrogenase from R. sphaeroides
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | OTHER |
Temperature [K] | 280 |
Detector technology | IMAGE PLATE |
Collection date | 1999-01-01 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 67.465, 67.465, 191.044 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
Rwork | 0.188 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1geg |
RMSD bond length | 0.005 * |
RMSD bond angle | 1.140 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.420 |
High resolution limit [Å] | 2.400 * | 2.420 * |
Rmerge | 0.078 * | 0.225 * |
Number of reflections | 21038 | |
<I/σ(I)> | 8 | |
Completeness [%] | 91.5 | 92.1 * |
Redundancy | 3 | 2.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7 | 300 | PEG4000, methanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | MES | 100 (mM) | pH7.0 |
3 | 1 | reservoir | PEG4000 | 18-21 (%) | |
4 | 1 | reservoir | methanol | 6-11 (%) |