1K1D
Crystal structure of D-hydantoinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 125 |
| Detector technology | CCD |
| Collection date | 2001-01-07 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.91 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 214.449, 182.890, 156.131 |
| Unit cell angles | 90.00, 125.27, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 3.000* |
| R-factor | 0.263 |
| Rwork | 0.263 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE (& GLRF) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.138 * | |
| Total number of observations | 943917 * | |
| Number of reflections | 96187 | |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 21 * | Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | reservoir | Tris-Cl | 100 (mM) | pH7.5 |
| 5 | 1 | reservoir | ammonium sulfate | 2.1 (M) |
