1K0N
Chloride Intracellular Channel 1 (CLIC1) complexed with glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.509, 60.355, 89.627 |
| Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
| Resolution | 91.290 - 1.800 |
| R-factor | 0.253 * |
| Rwork | 0.251 |
| R-free | 0.28500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.046 | 0.200 * |
| Number of reflections | 43638 | |
| <I/σ(I)> | 9.6 | 3.4 |
| Completeness [%] | 98.0 | 93.9 |
| Redundancy | 4.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5 | 4 * | PEG MME 5K, AMMONIUM SULPHATE, SODIUM ACETATE, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-20 (mg/ml) | |
| 2 | 1 | drop | n-octyl-beta-D-glucopyranoside | 0.5 (%) | |
| 3 | 1 | reservoir | PEG5000 MME | 14-18 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 0-30 (mM) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |
