1JZW

Arsenate Reductase + Sodium Arsenate From E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2000-09-07
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 61 2 2
Unit cell lengths	86.790, 86.790, 116.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.760
R-factor	0.163 *
Rwork	0.164
R-free	0.19200 *
Structure solution method	AB INITIO
RMSD bond length	0.012
RMSD bond angle	0.031 *
Data scaling software	X-GEN
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.107	0.330
Total number of observations	722598 *
Number of reflections	22762
<I/σ(I)>	21.232	8.51
Completeness [%]	84.8	40
Redundancy	31.74	8.02

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	5 *	50% saturated Cesium Sulfate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 278K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		1.2 (M)
2	1	drop	ammonium acetate	100 (mM)	pH4.8
3	1	drop	protein	30 (mg/ml)