1JYJ
Crystal Structure of a Double Variant (W67L/W91H) of Recombinant Human Serum Retinol-binding Protein at 2.0 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 1.09 |
Spacegroup name | H 3 |
Unit cell lengths | 102.762, 102.762, 72.725 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 * - 2.000 |
R-factor | 0.209 |
Rwork | 0.205 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jyd |
RMSD bond length | 0.006 |
RMSD bond angle | 1.379 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.102 * | 0.370 * |
Total number of observations | 109525 * | |
Number of reflections | 18890 | |
<I/σ(I)> | 7.53 | 3.19 |
Completeness [%] | 98.0 | 92.7 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 9.1 | 16 * | Sodium Chloride, Tris/HCl buffer, beta-octyl-glucoside, pH 9.1, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | beta-octylglucoside | 0.05 (%) | |
2 | 1 | drop | protein | 12 (mg/ml) | |
3 | 1 | reservoir | 4.5 (M) | ||
4 | 1 | reservoir | Tris-HCl | 50 (mM) | pH9.1 |
5 | 1 | reservoir | beta-octylglucoside | 0.05 (%) |