1JYJ
Crystal Structure of a Double Variant (W67L/W91H) of Recombinant Human Serum Retinol-binding Protein at 2.0 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.09 |
| Spacegroup name | H 3 |
| Unit cell lengths | 102.762, 102.762, 72.725 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 * - 2.000 |
| R-factor | 0.209 |
| Rwork | 0.205 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jyd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.379 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.102 * | 0.370 * |
| Total number of observations | 109525 * | |
| Number of reflections | 18890 | |
| <I/σ(I)> | 7.53 | 3.19 |
| Completeness [%] | 98.0 | 92.7 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 9.1 | 16 * | Sodium Chloride, Tris/HCl buffer, beta-octyl-glucoside, pH 9.1, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | beta-octylglucoside | 0.05 (%) | |
| 2 | 1 | drop | protein | 12 (mg/ml) | |
| 3 | 1 | reservoir | 4.5 (M) | ||
| 4 | 1 | reservoir | Tris-HCl | 50 (mM) | pH9.1 |
| 5 | 1 | reservoir | beta-octylglucoside | 0.05 (%) |
