1JX7

Crystal structure of ychN protein from E.coli

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-09
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97949, 0.97926, 0.99999
Spacegroup name	P 21 21 21
Unit cell lengths	66.210, 80.460, 140.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.800
R-factor	0.229
Rwork	0.229
R-free	0.24900 *
Structure solution method	MAD
RMSD bond length	0.008 *
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.100
High resolution limit [Å]	2.800	2.800 *
Rmerge	0.106 *	0.325 *
Number of reflections	35268 *	1756 *
Completeness [%]	97.3	98.6 *
Redundancy	3.07 *	2.93 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	21.5-22.5 *	AMS, pH 5.5, VAPOR DIFFUSION, HANGING DROP at 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	250 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)	pH7.2
3	1	drop		150 (mM)
4	1	drop	ammonium phosphate	2.4 (M)
5	1	drop	citric acid	0.1 (M)	pH5.5
6	1	reservoir	ammonium phosphate	2.4 (M)
7	1	reservoir	citric acid	0.1 (M)	pH5.5