1JX2
CRYSTAL STRUCTURE OF THE NUCLEOTIDE-FREE DYNAMIN A GTPASE DOMAIN, DETERMINED AS MYOSIN FUSION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2000-10-13 |
Detector | MARRESEARCH |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.450, 62.040, 181.200 |
Unit cell angles | 90.00, 94.79, 90.00 |
Refinement procedure
Resolution | 14.960 - 2.300 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g8x MYOSIN II CATALYTIC DOMAIN |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.050 * | 0.215 * |
Number of reflections | 52742 | |
<I/σ(I)> | 19.3 | 4.98 |
Completeness [%] | 97.9 | 94.6 |
Redundancy | 3.7 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | PEG 8000, Tris, potassium chloride, magnesium chloride, glucose, methyl-propane-diol, dithiothreitol, EGTA, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 11 (%) | |
10 | 1 | drop | 1 (mM) | ||
11 | 1 | drop | dithiothreitol | 1 (mM) | |
12 | 1 | drop | sucrose | 3 (%) | |
13 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | Tris | 50 (mM) | |
3 | 1 | reservoir | 200 (mM) | ||
4 | 1 | reservoir | 5 (mM) | ||
5 | 1 | reservoir | glucose | 10 (%) | |
6 | 1 | reservoir | MPD | 2 (%) | |
7 | 1 | reservoir | EGTA | 1 (mM) | |
8 | 1 | reservoir | dithiothreitol | 5 (mM) | |
9 | 1 | drop | Tris | 50 (mM) |